Recombinant Biotinylated Human PD-L2/B7-DC Protein,His-Avi Tag

Description :
Source Biotinylated Human PD-L2/B7-DC Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Leu20-Thr220.[Accession |Q9BQ51-1] Molecular Weight The protein has a predicted MW of 25.3 kDa. Due to glycosylation, the protein migrates to 40-52 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity SPR Data:Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-L2, His-Avi Tag with an affinity constant of 0.344 M as determined in SPR assay. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.