Recombinant Biotinylated Human B7-H2/ICOSLG Protein,His-Avi Tag

Description :
Source Biotinylated Human B7-H2/ICOSLG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Asp19-Ser258.[Accession |O75144] Molecular Weight The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data:Immobilized Human ICOS (C136S,C137S) , His Tag at 1g/ml (100l/Well) on the plate. Dose response curve for Biotinylated Human B7-H2, His Tag with the EC50 of 23.9ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.