Recombinant Biotinylated Human DNAM-1/CD226 Protein,His-Avi Tag

Description :
Source Biotinylated Human DNAM-1/CD226 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu19–Asn247.[Accession |Q15762] Molecular Weight The protein has a predicted MW of 28.9 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandSEC-HPLC Activity SPR Data: Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human DNAM-1, His-Avi Tag with an affinity constant of 264nM as determined in SPR assay. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.