Recombinant Biotinylated Human IFN alpha/beta R1 Protein,His-Avi Tag

Description :
Source Biotinylated Human IFN alpha/beta R1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Lys28-Lys436.[Accession |P17181-1] Molecular Weight The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Anti-IFNAR1 Antibody, hFc Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human IFNAR1, His Tag with the EC50 of 1.7ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.