Recombinant Biotinylated Human IL-23 alpha&IL-12 beta Protein,His-Avi Tag

Description :
Source Biotinylated Human IL-23 alpha&IL-12 beta Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Arg20-Pro189(IL-23 alpha)&Ile23-Ser328(IL-12 beta).[Accession |Q9NPF7(IL-23 alpha)&P29460(IL-12 beta)] Molecular Weight The protein has a predicted MW of 21.7 (IL-23 alpha)&34.7 (IL-12 beta) kDa. Due to glycosylation,the protein migrates to 24 kDa (IL-23 alpha) and 40-50 kDa (IL-12 beta) kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGE Activity ELISA Data:Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 0.5g/ml (100l/Well) on streptavidin (5g/ml) precoated plate. Dose response curve for Anti-IL-23 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.