Recombinant Human APOE4/Apolipoprotein E Protein,HisTag

Description :
蛋白别名Synonyms Apo-E;Apolipoprotein E; APOE 表达系统及表达区间Source Human APOE4/Apolipoprotein E Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Lys19-His317.[Accession |AAB59397.1] 分子量Molecular Weight The protein has a predicted MW of 61.6 kDa. Due to glycosylation, the protein migrates to 63-67 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGE Formulation Lyophilized from 0.22m filtered solution in PBS, 2mM DTT, 5mM CHAPS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 复溶方法Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.