Recombinant Mouse tPAProtein,His Tag

Description :
Synonyms t-PA; tPA; Alteplase; Reteplase; PLAT; Source Mouse tPAProtein is expressed from HEK293 with His tag at the C-Terminus. It contains Ser33-Gln559, which consists of two chains: chain A (Ser33-Arg308) and chain B (Ile309-Gln559).[Accession | P11214] Molecular Weight The protein has a predicted MW of 60.57 kDa. It migrates to 13-15 kDa (chain A and chain B fragment), 30-34 kDa and 35-40 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.