Recombinant Human IL-27 Protein,His Tag

Description :
Synonyms IL30;IL-27A;p28;Interleukin-27 Source Human IL-27 Protein is expressed from CHO with His tag at the C-Terminus. It contains Arg21-Lys229(IL-27B)&Phe29-Pro243(IL-27A).[Accession | Q14213(IL-27B)&Q8NEV9(IL-27A)] Molecular Weight The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 54-64 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human IL-27 RA, hFc Tag at 5g/ml (100l/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 55.9ng/ml determined by ELISA . Formulation Lyophilized from 0.22m filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.