Recombinant Mouse NKG2D/CD314 Protein,His Tag

Description :
Synonyms KLR; D12S2489E;CD314; NKG2D Source Mouse NKG2D/CD314 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Phe90-Val232.[Accession | O54709-1] Molecular Weight The protein has a predicted MW of 17.5 kDa. Due to glycosylation, the protein migrates to 24-35kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Mouse NKG2D, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 0.39g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in 20mM Tris, 250mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.