Recombinant Human Galectin 3 Protein,His Tag

Description :
Synonyms GAL3; CBP35;AGE-R3; galactin-3 Source Human Galectin 3 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Ala2-Ile250.[Accession | P17931] Molecular Weight The protein has a predicted MW of 27.1 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human Galectin 3, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 15.0ng/ml determined by ELISA . Formulation Lyophilized from 0.22m filtered solution in 50mM HEPES, 150mM NaCl, 5mM EDTA, 2mM TCEP (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.