Recombinant Biotinylated Human B7-1/CD80 Protein,His-Avi Tag

Description :
Synonyms B71; B7;CD80 molecule; BB1 Source Biotinylated Human B7-1/CD80 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Val35-Asn242.[Accession | P33681-1] Molecular Weight The protein has a predicted MW of 26.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Biotinylated Human B7-1, His Tag at 0.5g/ml (100l/Well) on streptavidin (5g/ml) precoated plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.