Recombinant Biotinylated Cynomolgus BTN3A1/CD277 Protein,His-Avi Tag

Description :
Synonyms BTF5; BTN3A1;CD277; BTN3.1 Source Biotinylated Cynomolgus BTN3A1/CD277 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gln1-Trp219.[Accession | A0A330KWZ1] Molecular Weight The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Anti-BTN3A1 Antibody, hFc Tag at 1g/ml (100l/well) on the plate. Dose response curve for Biotinylated Cynomolgus BTN3A1, His Tag with the EC50 of 14.2ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.