Recombinant Human KLKB1 Protein,His Tag

Description :
Synonyms KLK3; PKK;Plasma kallikrein; Fletcher factor Source Human KLKB1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gly20-Ala638.[Accession | P03952] Molecular Weight The protein has a predicted MW of 70.3 kDa. Due to glycosylation, the protein migrates to 72-80 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human KLKB1, His Tag at 2g/ml (100l/well) on the plate. Dose response curve for Anti-KLKB1 Antibody, hFc Tag with the EC50 of 33.8ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in 20mM NaAc,150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc,150mM NaCl (pH 5.0).