Recombinant Biotinylated Human IL-10 Protein,His-Avi Tag

Description :
Synonyms Interleukin-10; IL10A; CSIF; B-TCGF Source Biotinylated Human IL-10 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Ser19-Asn178.[Accession | P22301] Molecular Weight The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data: Human IL-10 R alpha, His Tag immobilized on CM5 Chip can bind Biotinylated Human IL-10, His Tag with an affinity constant of 0.30 nM as determined in SPR assay . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.