Recombinant Human LRP-6Protein,mFc Tag

Description :
Synonyms FLJ90062; DCAD2;LRP-6;LRP6 Source Human LRP-6Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus. It contains Ala20-Pro630.[Accession | O75581-1] Molecular Weight The protein has a predicted MW of 95.3 kDa. Due to glycosylation, the protein migrates to 110-115 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 90% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human LRP-6, mFc Tag at 5g/ml (100l/Well) on plate. Dose response curve for Biotinylated Human SOST, His Tag with the EC50 of 0.87g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.