Recombinant Biotinylated Human IL-4 Protein,His-Avi Tag

Description :
Synonyms interleukin 4; IL-4;IL4; pitrakinra Source Biotinylated Human IL-4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains His25-Ser153.[Accession | P05112-1] Molecular Weight The protein has a predicted MW of 17.8 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human IL-4 R alpha, hFc Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Biotinylated Human IL-4, His Tag with the EC50 of 5.0ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.