Recombinant Biotinylated Human Nectin-1/PVRL1/CD111 Protein,His-Avi Tag

Description :
Synonyms CD111; HVEC; HIgRPVRR; Nectin1 Source Biotinylated Human Nectin-1/PVRL1/CD111 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gln31-Thr334.[Accession | Q15223-1] Molecular Weight The protein has a predicted MW of 36.9 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data: Human Nectin-4, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-1, His-Avi Tag with an affinity constant of 22.83 nM as determined in SPR assay . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.