Recombinant Human TNFSF15 Trimer Protein,His-Flag Tag

Description :
Synonyms TNFSF15; VEGI-251;TL1A; VEGI Source Human TNFSF15 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus. It contains Asp91-Leu251.[Accession | O95150-1] Molecular Weight The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human TNFSF15 Trimer, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 10.2ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.