Recombinant Biotinylated Human IL-15RA&IL-15 Protein (Primary Amine Labeling),His Tag

Description :
Synonyms IL15RA;CD215;IL-15 R alpha;IL-15R-alpha1 Source Biotinylated Human IL-15RA&IL-15 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus. It contains Ile31-Ser108(IL-15RA)&Asn49-Ser162(IL-15).[Accession | Q13261-1(IL-15RA)&P40933-1(IL-15)] Molecular Weight The protein has a predicted MW of 23.8 kDa. Due to glycosylation, the protein migrates to 25-28 kDa&30-35 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human IL-2 R beta, hFc Tag at 1g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human IL-15Ra&IL-15, His Tag with the EC50 of 18.5ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.