Recombinant Mouse GFRAL/GFR alpha-like Protein,His-Avi Tag

Description :
Synonyms GRAL; GFRAL;GFR alpha-like; Source Mouse GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Gln20-Glu350.[Accession | Q6SJE0-1] Molecular Weight The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Mouse GFRAL, His Tag at 2g/ml (100l/well) on the plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.