Recombinant Cynomolgus APRIL/TNFSF13 Trimer Protein,His-Avi-Flag Tag

Description :
Synonyms TALL-2; TALL2;CD256; APRIL Source Cynomolgus APRIL/TNFSF13 Trimer Protein is expressed from HEK293 with His tag and Flag tag and Avi tag at the N-Terminus. It contains Lys112-Leu250.[Accession | A0A2K5TJA1] Molecular Weight The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Cynomolgus APRIL (Trimer) , His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Cynomolgus BCMA, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in 50mM Tris, 150mM NaCl, 200mM L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.