Recombinant Human IL-1R1 Protein,His Tag

Description :
Synonyms IL-1R-alpha; IL-1RT1;IL-1R-1; IL-1 RI Source Human IL-1R1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Leu18-Thr332.[Accession | NP_000868.1] Molecular Weight The protein has a predicted MW of 37 kDa. Due to glycosylation, the protein migrates to 55-68 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human IL-1R1, His Tag at 2g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 0.13g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.