Recombinant Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein,His-Avi Tag

Description :
Synonyms ITGA5&ITGB1; VLA-5;Integrin alpha 5 beta 1; 51 Source Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Phe42-Tyr995(ITGA5) acidic tail and Gln21-Asp728(ITGB1) basic tail.[Accession | P08648(ITGA5)&P05556-1(ITGB1)] Molecular Weight The protein has a predicted MW of 111.8 kDa (ITGA5)&83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 100-140 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human ITGA5&ITGB1, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Anti-ITGA5&ITGB1 Antibody, hFc Tag with the EC50 of 16.2ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.