Recombinant Mouse ARTN Protein,hFc Tag

Description :
Synonyms Neublastin; Enovin;Artemin; EVN Source Mouse ARTN Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Ala112-Gly224.[Accession | Q9Z0L2-1] Molecular Weight The protein has a predicted MW of 39.4 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Mouse GFRA3, His Tag at 5g/ml (100l/Well) on the plate. Dose response curve for Mouse ARTN, hFc Tag with the EC50 of 0.12g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in 50mM Glycine,150mM NaCl (pH 3.2). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in 50mM Glycine,150mM NaCl (pH 3.2).