Recombinant Biotinylated Human IL-1 Beta/IL-1F2 Protein,His-Avi Tag

Description :
Synonyms IL1F2; IL-1 beta;Interleukin-1 beta; IL-1BETA Source Biotinylated Human IL-1 beta/IL-1F2 Protein is expressed from E.coil with His tag and Avi tag at the C-Terminus. It contains Ala117-Ser269.[Accession | P01584] Molecular Weight The protein has a predicted MW of 20.1 kDa same as SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human IL-1R2, hFc Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human IL-1 beta, His Tag with the EC50 of 13.1ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.