Recombinant Human APLN Protein,hFc Tag

Description :
Synonyms APEL;APJ endogenous ligand;Apelin; Source Human APLN Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gly23-Phe77.[Accession | Q9ULZ1] Molecular Weight The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human APLN, hFc Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Biotinylated Anti-APLN Antibody, hFc Tag with the EC50 of 22.2ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS, 200mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.