Recombinant Human IL-31 RAProtein,hFc Tag

Description :
Synonyms Gp130-like receptor; IL-31R-alpha;IL-31RA; GPL Source Human IL-31 RAProtein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ala20-Glu519.[Accession | Q8NI17-1] Molecular Weight The protein has a predicted MW of 83.8 kDa. Due to glycosylation, the protein migrates to 110-135 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human IL-31 RA, hFc Tag at 5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Anti-IL-31 RA Antibody, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA .Immobilized Human IL-31, His Tag at 5g/ml (100l/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.15g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.