Recombinant Mouse Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein,His Tag

Description :
Synonyms Integrin alpha V beta 6; ITGAV&ITGB6;V6; Source Mouse Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Phe31-Val988(ITGAV) acidic tail&Gly22-Asn706(ITGB6) basic tail.[Accession | P43406(ITGAV)&Q9Z0T9(ITGB6)] Molecular Weight The protein has a predicted MW of 111.6 kDa (ITGAV)&79.2 kDa (ITGB6). Due to glycosylation, the protein migrates to 90-140 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Mouse ITGAV&ITGB6, His Tag at 1g/ml (100l/Well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 41.8ng/ml determined by ELISA .Immobilized Mouse ITGAV&ITGB6, His Tag at 2g/ml (100l/well) on the plate. Dose response curve for Biotinylated Mouse GARP&Latent TGF beta 1 Complex, His Tag with the EC50 of 89.3ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.