Recombinant Cynomolgus MMP-9 Protein,His Tag

Description :
Synonyms CLG4B; GELB;MMP-9; Gelatinase B Source Cynomolgus MMP-9 Protein is expressed from HEK293 with His tag at the C-Terminus. The protein needs to be activated by APMA to have hydrolytic activity. It contains Ala20-Asp706.[Accession | A0A2K5UU71] Molecular Weight The protein has a predicted MW of 77.44 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Cynomolgus MMP-9, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.