Recombinant Biotinylated Human Alkaline Phosphatase (Germ type) /ALPG Protein,His-Avi Tag

Description :
Synonyms Alkaline Phosphatase; ALP-1; Alkaline Phosphatase, placental-like; GCAP; PLAP-like; ALPG; ALPPL; ALPPL2 Source Biotinylated Human Alkaline Phosphatase (Germ type) /ALPG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ile20-Asp503.[Accession | P10696] Molecular Weight The protein has a predicted MW of 55.48 kDa. Due to glycosylation, the protein migrates to 56-70 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE,> 90% as determined by HPLC Activity ELISA Data: Immobilized Anti-Alkaline Phosphatase Antibody, hFc Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human Alkaline Phosphatase (Germ type) , His Tag with the EC50 of 23.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.