Recombinant Human&Mouse Chimeric WT-1 (HLA-A*02:01) Complex Protein,His-Avi Tag

Description :
Source Recombinant Human&Mouse Chimeric WT-1 (HLA-A*02:01) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Gly25-Thr206(Human HLA-A*02:01 1&2)&Asp207-Ala362(Mouse HLA-A*02:01 3), Ile21-Met119 (B2M) and RMFPNAPYL peptide.[Accession|A0A140T913(Human HLA-A*02:01 1&2) &P01897(Mouse HLA-A*02:01 3)&P61769(B2M)&RMFPNAPYL] Molecular Weight The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per ug by the LAL method. Purity > 95% as determined by Tris-Bis PAGE > 95% as determined by HPLC Activity ELISA Data: Immobilized Human&Mouse Chimeric WT-1 (HLA-A*02:01) , His Tag at 5g/ml (100l/well) on the plate. Dose response curve for Anti-WT-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 28.1ng/ml determined by ELISA. Formulation Supplied as 0.22m filtered solution in PBS (pH 7.4).