Recombinant Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein,hFc-Avi Tag

Description :
Source Recombinant Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with hFc tag and Avi tag at the C-terminal. It contains Asp23-Asp126(CD3E) & Gln23-Ser116(CD3G).[Accession |P07766(CD3E)&P09693(CD3G)] Molecular Weight The protein has a predicted MW of 40 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation, the protein migrates to 48-55 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGE > 95% as determined by HPLC Activity ELISA Data: Immobilized Biotinylated Human CD3E&CD3G, hFc Tag at 1g/ml (100l/Well) on streptavidin (5g/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 30.3ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.