Recombinant Biotinylated Human CD47 Protein,His-Avi Tag

Description :
Source Biotinylated Human CD47 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains GIn19-Pro139.[Accession |Q08722-1] Molecular Weight The protein has a predicted MW of 16.63 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Human SIRP alpha, hFc Tag at 1g/ml (100l/Well) on the plate. Dose response curve for Biotinylated Human CD47, His Tag with the EC50 of 0.48g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.