Recombinant Human CD28H/IGPR-1 Protein,hFc Tag

Description :
Source Human CD28H/IGPR-1 Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Leu23-Gly150.[Accession |Q96BF3-1] Molecular Weight The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEand HPLC Activity ELISA Data: Immobilized Human B7-H7, His Tag at 2g/ml (100l/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 0.89g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.