Recombinant Biotinylated Human Nectin-3/CD113 Protein,His-Avi Tag

Description :
Source Biotinylated Human Nectin-3/CD113 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Gly58-Asp400.[Accession |Q9NQS3-1] Molecular Weight The protein has a predicted MW of 40.6 kDa. Due to glycosylation, the protein migrates to 68-82 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity SPR Data: Biotinylated Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.13 M as determined in SPR assay Formulation Lyophilized from 0.22m filtered solution in 20mM PB, 250mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.