Recombinant Canine CD40/TNFRSF5 Protein,His Tag

Description :
Synonyms CD40; CD40L receptor; TNFRSF5; CD40 antigen; CD40 molecule; CDw40; MGC9013; Source Canine CD40/TNFRSF5 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu21-Ala194.[Accession | Q7YRL5] Molecular Weight The protein has a predicted MW of 20.14 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Canine CD40, His Tag at 5g/ml (100l/well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.