Recombinant Biotinylated Human TPBG/5T4 Protein,His-Avi Tag

Description :
Source Biotinylated Human TPBG/5T4 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ser32-Ser355.[Accession |Q13641-1] Molecular Weight The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Anti-TPBG Antibody, hFc Tag at 2g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human TPBG, His Tag with the EC50 of 43.3ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.