Recombinant Human TRAIL R4/TNFRSF10D Protein,His-Avi Tag

Description :
Source Human TRAIL R4/TNFRSF10D Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ala56-His211.[Accession |Q9UBN6] Molecular Weight The protein has a predicted MW of 19.4 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEand HPLC Activity ELISA Data: Immobilized Human TRAIL, No Tag at 5g/ml (100l/well) on the plate. Dose response curve for Human TRAIL R4, His Tag with the EC50 of 76.1ng/ml determined by ELISA. SPR Data: Human TRAIL R4, His Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.13 nM as determined in SPR assay Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.