Recombinant Biotinylated Human IL-18BP Protein,His-Avi Tag

Description :
Source Biotinylated Human IL-18BP Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Thr31-Gly194.[Accession |O95998-1] Molecular Weight The protein has a predicted MW of 20.5 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity SPR Data: Biotinylated Human IL-18BP, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 0.90 nM as determined in SPR assay Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.