Recombinant Human Alkaline Phosphatase Protein (Placental type),His Tag

Description :
Source Human Alkaline Phosphatase Protein is expressed from HEK293 with His tag at the C-terminal. It contains Ile23-Asp506.[Accession |P05187] Molecular Weight The protein has a predicted MW of 53.8 kDa. Due to glycosylation, the protein migrates to 63-68 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per ug by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Human Alkaline Phosphatase, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 23.0ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.