Recombinant Biotinylated Human MMP-9 Protein (pro form) ,His-Avi Tag

Description :
Source Biotinylated Human MMP-9 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala20-Asp707.[Accession |P14780] Molecular Weight The protein has a predicted MW of 79.3 kDa. Due to glycosylation, the protein migrates to 95-105 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEand HPLC Activity ELISA Data: Immobilized Anti-MMP-9 Antibody, hFc Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human MMP-9, His Tag with the EC50 of 14.3ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.