Recombinant Biotinylated Human GFRAL/GFR alpha-like Protein,His-Avi Tag

Description :
Synonyms GFR alpha-like; GFRAL; GRAL; C6orf144 Source Biotinylated Human GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ser19-Glu351.[Accession |Q6UXV0] Molecular Weight The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 48-62 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEand HPLC Activity ELISA Data: Immobilized Human GDF15, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human GFRAL, His Tag with the EC50 of 89.1ng/ml determined by ELISA BlockingData: Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8ng/ml. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.