Recombinant Biotinylated Mouse GARP&Latent TGF beta 1 Complex Protein,His-Avi Tag

Description :
Synonyms LAP; GARP;LRRC32; LRRC32&TGF-beta 1 Source Biotinylated Mouse GARP&Latent TGF beta 1 Complex Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Ala18-Leu628(GARP)&Leu30-Ser390(Latent TGF beta 1).[Accession | G3XA59(GARP)&P04202(Latent TGF beta 1)] Molecular Weight The protein has a predicted MW of 66.6 kDa (GARP)&44.2 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 72-76 kDa (GARP)&13 kDa&45-47 kDa (Latent TGF beta 1) based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Mouse ITGAV&ITGB6, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Biotinylated Mouse GARP&Latent TGF beta 1 Complex, His Tag with the EC50 of 0.12g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.