Recombinant Biotinylated Mouse Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein,His-Avi Tag

Description :
Synonyms V3; Integrin alpha V beta 3; beta3; alphaV; ITGAV&ITGB3;Integrin alpha V 3; 3 Source Biotinylated Mouse Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Phe31-Val988(ITGAV) acidic tail & Glu26-Asp717(ITGB3) basic tail.[Accession | P43406(ITGAV)&O54890(ITGB3)] Molecular Weight The protein has a predicted MW of 108.77 kDa(ITGAV)&80.84 kDa(ITGB3). Due to glycosylation, the protein migrates to 90-130 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human Vitronectin, His Tag at 5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Mouse ITGAV&ITGB3, His Tag with the EC50 of 3.2g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.