Recombinant Cynomolgus PD-L2/B7-DC Protein,His Tag

Description :
Synonyms PDL2; CD273, PDCD1 ligand 2; Butyrophilin B7-DC; PDCD1LG2 Source Cynomolgus PD-L2/B7-DC Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Leu20-Thr220.[Accession | XP_005581839] Molecular Weight The protein has a predicted MW of 23.73 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data: Cynomolgus PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus PD-L2, His Tag with an affinity constant of 0.17 M as determined in SPR assay . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.