Recombinant Biotinylated Human SOST/Sclerostin Protein,His-Avi Tag

Description :
Synonyms VBCHsclerosteosis; SOST;sclerostin; DAND6 Source Biotinylated Human SOST/Sclerostin Protein is expressed from HEK293 with His and Avi tag at the N-Terminus. It contains Gln24-Tyr213.[Accession | Q9BQB4-1] Molecular Weight The protein has a predicted MW of 24.2 kDa. Due to glycosylation,the protein migrates to 28-40 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Anti-SOST Antibody at 0.5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human SOST, His Tag with the EC50 of 3.9ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.