Recombinant Human B7-2/CD86 Protein,His-Avi Tag

Description :
Synonyms B70; CD86;CD86 molecule; B72 Source Human B7-2/CD86 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Leu26-Pro247.[Accession | P42081-1] Molecular Weight The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human B7-2, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 0.12g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.