Recombinant Rat GDF15 Protein,His Tag

Description :
Synonyms NAG-1; MIC-1;GDF-15; PLAB Source Rat GDF15 Protein is expressed from E.coli with His tag at the N-Terminus. It contains Ser189-Ala303.[Accession | Q9Z0J6] Molecular Weight The protein has a predicted MW of 13.68 kDa. The protein migrates to 15-17 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Rat GDF-15, His Tag at 2g/ml (100l/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA .Immobilized Rat GDF15, His Tag at 5g/ml (100l/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.95g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).