Recombinant Biotinylated Human GITR Ligand/TNFSF18 Trimer Protein (Primary Amine Labeling),His-Flag Tag

Description :
Synonyms TL6; AITRL;TNFSF18; GITR Ligand Source Biotinylated Human GITR Ligand/TNFSF18 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with His tag and flag tag at the N-Terminus. It contains Gln50-Ser177.[Accession | Q9UNG2] Molecular Weight The protein has a predicted MW of 47.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human GITR, hFc Tag at 2g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 11.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.