Recombinant Human CTGF/CCN2 Protein,hFc Tag

Description :
Synonyms CCN2; IBP-8;IGFBP8; HCS24 Source Human CTGF/CCN2 Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Gln27-Ala349.[Accession | Q5M8T4] Molecular Weight The protein has a predicted MW of 61.77 kDa. Due to glycosylation, the protein migrates to 50-55 kDa and 62-72 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human CTGF, hFc Tag at 0.2g/ml (100l/Well) on the plate. Dose response curve for Biotinylated Anti-CTGF Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.